Table 1 Basic characteristics of the 19 eligible studies
Study | Design |  N  |  KRAS  mutation | Agent; treatment modality | Line | Response criteria | Tumor sample | GCN assay |
|---|---|---|---|---|---|---|---|---|
Bengala 200925 | Retrospective | 55 | 36.5 % | Cmab/Pmab; CT | NA | NA | NA | FISH |
Campanella 201026 | Retrospective | 88 | 37.7 % | Cmab; mixed | Mixed | RECIST | NA | FISH |
Cappuzzo 200823 | Retrospective | 85 | NA | Cmab; CT | ≥2nd | RECIST | Mixed | FISH |
Frattini 200727 | Retrospective | 27 | 37.0 % | Cmab; CT | Mixed | RECIST | Primary | FISH |
Gevorgyan 200728 | Retrospective | 40 | NA | Cmab; CT | NA | NA | Primary | FISH |
Goncalves 200829 | Retrospective | 29 | 44.8 % | Cmab; CT | ≥2nd | WHO | Mixed | FISH |
Italiano 200815 | Retrospective | 41 | NA | Cmab; CT | NA | RECIST | Primary | FISH |
Khambata-Ford 200730 | Prospective | 56 | 34.6 % | Cmab; MT | ≥2nd | WHO | Metastatic | Q-PCR |
Laurent-Puig 200916 | Retrospective | 96 | 0 % | Cmab; CT | ≥2nd | RECIST | Mixed | FISH + CISH |
Lievre 200631 | Retrospective | 30 | 43.3 % | Cmab; CT | ≥2nd | RECIST | Primary | CISH |
Mancuso 200832 | Retrospective | 31 | 32.3 % | Cmab; NA | NA | NA | NA | FISH |
Moroni 200514 | Retrospective | 29 | 31.0 % | Cmab/Pmab; mixed | Mixed | RECIST | Primary | FISH |
Perrone 200924 | Retrospective | 31 | 24.1 % | Cmab; CT | ≥2nd | RECIST | Mixed | FISH |
Personeni 200833 | Retrospective | 87 | 33.3 % | Cmab; mixed | ≥3rd | RECIST | NA | FISH |
Razis 200834 | Retrospective | 66 | NA | Cmab; CT | ≥2nd | NA | Metastatic | FISH |
Sartore-Bianchi 200717 | Retrospective | 58 | NA | Pmab; MT | ≥2nd | RECIST | NA | FISH |
Sastre 200935 | Prospective | 36 | 21.7 % | Cmab; MT | 1st | WHO | NA | FISH |
Scartozzi 200936 | Retrospective | 37 | 0 % | Cmab; CT | ≥2nd | RECIST | Primary | FISH |
Tol 201037 | Retrospective | 155 | 0 % | Cmab; CT | 1st | RECIST | Primary | FISH |
Study | Criteria for GCN+ | GCN + % | ||||||
Bengala 200925 | Gene copies/nucleus ≥ 2.9 | 30.9 | ||||||
Campanella 201026 | Polysomy: CEP7 ≥ 3; amplification: gene/CEP7 ≥ 2.0 | 68.2 | ||||||
Cappuzzo 200823 | Gene copies/nucleus ≥2.92* | 50.6 | ||||||
Frattini 200727 | High polysomy: CEP7 > 4 in ≥ 50 % of cells; amplification: gene/CEP7 > 3 in ≥ 10 % of cells | 88.9 | ||||||
Gevorgyan 200728 | High polysomy: ≥ 4 gene copies in ≥ 40 % of cells; amplification: gene clusters, gene/CEP7 ≥ 2, or ≥ 15 gene copies in ≥ 10 % of cells | 20.0 | ||||||
Goncalves 200829 | High polysomy: ≥ 4 gene copies in ≥ 40 % of cells and gene/CEP7 ≤ 2; amplification: gene/CEP7 ≥ 2 or ≥ 15 gene copies in ≥ 10 % of cells | 6.9 | ||||||
Italiano 200815 | High polysomy: ≥ 4 gene copies in ≥ 40 % of cells; amplification: gene/CEP7 ≥ 2, or ≥ 15 gene copies in ≥ 10 % of cells | 19.5 | ||||||
Khambata-Ford 200730 | NA | 7.1 | ||||||
Laurent-Puig 200916 | High polysomy: ≥ 4 gene copies in ≥ 40 % of cells; amplification: gene clusters, gene/CEP7 ≥ 2, or ≥ 10 gene copies in ≥10 % of cells | 17.7 | ||||||
Lievre 200631 | Amplification: ≥ 6 gene copies in > 50 % of cells, or gene clusters | 10.0 | ||||||
Mancuso 200832 | Gene copies/nucleus ≥ 3 | 58.1 | ||||||
Moroni 200514 | Gene copies/nucleus ≥ 3 | 31.0 | ||||||
Perrone 200924 | Gene copies/nucleus ≥ 2.79* | 74.2 | ||||||
Personeni 200833 | Gene copies/nucleus ≥ 2.83* | 37.9 | ||||||
Razis 200834 | gene/CEP7 > 1.2 | 7.6 | ||||||
Sartore-Bianchi 200717 | Polysomy: gene copies/nucleus ≥ 3; amplification: gene/CEP7 ≥ 2; in ≥ 43 % of cells | 32.8 | ||||||
Sastre 200935 | High polysomy: ≥ 4 gene copies in ≥ 40 % of cells; amplification: gene/CEP7 ≥ 2, or ≥ 15 gene copies in ≥ 10 % of cells | 30.6 | ||||||
Scartozzi 200936 | Gene copies/nucleus ≥ 2.6* | 40.5 | ||||||
Tol 201037 | Gene copies/nucleus ≥ 3, or gene/CEP7 ≥ 2 | 15.3 | ||||||