CDF upregulates the expression of miR-34a and miR-34c in different colon cancer cell lines. (A) The miR-34a and (B) miR-34c expression was increased significantly in HCT116CR, HCT116p53−/− cells following treatment with CDF. (C) The miR-34a and (D) miR-34c expression was also up-regulated significantly in SW620 cells treated with either CDF or Aza-dC for 72 h. ( DMSO, Aza-dC, CDF in the figure are DMSO, 5 μM of 5-Aza-2'- deoxycytidine, 100nM of CDF treated cells. * P, ** P denote the level of significance in Aza-dC or CDF treated cells, compared to DMSO-treated controls). The quantitative values were expressed as means ± SD of triplicate measurements, and are representative of two separate experiments.