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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: Bioluminescence imaging of leukemia cell lines in vitro and in mouse xenografts: effects of monoclonal and polyclonal cell populations on intensity and kinetics of photon emission

Figure 3

Generation of monoclonal luciferase-transduced human leukemia cell lines. Monoclonal luciferase-transduced Jurkat cell lines (wildtype, shControl, shMer1A and shMer1B) were isolated and bioluminescence intensity was determined as an indicator of luciferase activity (Bining: 8, FOV 13.2, f/stop 1, exposure time 5 sec). Four Jurkat wildtype clones, nine shControl clones, six shMer1A clones and four shMer1B clones were developed and analyzed. Jurkat wildtype clone 3, shControl clone 9 and shMer1A clone 1 had equal levels of luciferase activity and were selected and used for further studies.

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Journal of Hematology & Oncology

ISSN: 1756-8722

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