Figure 5
Longitudinal quantitation of bioluminescence signals in mice transplanted with monoclonal luciferase-transduced human leukemia cell lines. a NSG mice were transplanted with 5 x 106 monoclonal luciferase-transduced wildtype Jurkat cells by injection into the tail vein. D-luciferin was injected intraperitoneally and bioluminescence images were taken twice weekly (Bining: 8, FOV 19.6, f/stop 1, exposure time 120 sec). Pseudocolor images of mice transplanted with monoclonal luciferase-expressing human Jurkat wildtype cells showing equal development of bioluminescence intensity over time. b Quantitation of bioluminescence signal from each animal plotted against the number of days after transplantation. c Pseudocolor images of two representative mice transplanted with monoclonal luciferase-expressing human leukemia cells (Jurkat, 697 and K562) showing equal development of bioluminescence intensity and demonstrating establishment of leukemia in different organs.