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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Abnormal hematopoietic phenotypes in Pim kinase triple knockout mice

Figure 2

Spleen weight, total BM cell number, and CFUs in Pim TKO mice. (A) Reduced spleen weight in Pim TKO mice. Spleens from Pim TKO mice and WT controls were isolated and weighted. Data shown are the percentage of spleen weight to total body weight (n=5, **p < 0.01). (B) Reduced total bone marrow cells in Pim TKO mice. Bone marrow cells from 2 femurs and 2 tibias were harvested from Pim TKO mice and age-matched WT mice, depleted of RBCs, and counted (n=7, *p < 0.05). (C) Reduced total splenocytes in Pim TKO mice. Spleens from Pim TKO mice and age-matched WT mice were harvested, depleted of RBCs and counted (n=5, **p < 0.01). (D) Reduced CFUs in Pim TKO mice. BM cells were harvested from Pim TKO mice or WT controls and plated in Methocult® GF 3434 (1x105/dish in triplicate). The numbers of CFUs-GM, BFUs-E and CFUs-GEMM were counted at day 7, day 9, and day 12, respectively (n=4, *p < 0.05, **p<0.01). (E) Comparable percentages of LSK cells in Pim TKO mice. RBC-depleted BM cells were analyzed for LSK cell population. Data shown represent fold change over WT controls (n = 7). (F) Reduced absolute number of BM LSK cells in Pim TKO mice. The absolute LSK cells were calculated by multiplying the total number of BM cells (from 2 femurs and 2 tibias) with the percentage of LSK cells (n = 3, *p < 0.05 data representative of 3 independent experiments). (G) Unchanged percentage of LT-HSCs, ST-HSCs, and MPPs in Pim TKO mice. Bone marrow cells were harvested, depleted of RBCs and enriched for Lin- cells using lineage depletion kit. The enriched Lin- cells were then stained with CD34, CD135, Sca-1, and c-Kit antibodies as described in the Materials and Methods. Data represent fold changes over WT control mice (n=5).

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