Epigenetic inactivation of the MIR129-2 in hematological malignancies

Table 2 Primer sequences and reaction conditions

Gene	Forward primer (5’ – 3’)	Reverse primer (5’ – 3’)	Product size (bp)	Tm/cycles
(I) Methylation-specific polymerase chain reaction (MSP)
MIR129-2
M-MSP	GAGTTGGGGGATCGCGGAC	ATATACCGACTTCTTCGATTCGCCG	188	59°C/ 35
U-MSP	GAGTTGGGGGATTGTGGAT	AATATACCAACTTCTTCAATTCACCA	189	55°C/ 35
(II) Reverse transcription-polymerase chain reaction (RT-PCR)
SOX4	GCTGGAAGCTGCTCAAAGAC	ACCGACCTTGTCTCCCTTCT	167	60°C/ 40
GAPDH	ACCACAGTCCATGCCATCACT	TCCACCACCCTGTTGCTGTA	452	60°C/ 40

Keys: M-MSP, MSP for the methylated allele; Tm, annealing temperature; U-MSP, MSP for the unmethylated allele.

ISSN: 1756-8722