Skip to main content

Table 2 Comparison of different detection methods of mtROS

From: Targeting mitochondrial reactive oxygen species as novel therapy for inflammatory diseases and cancers

Methods Drugs Detected species Principle Limits
Fluorescent probes MitoSOX Superoxide Attaching a hydrophobic cation to dihydroethidium. Two fluorescent products, one of which is superoxide specific. The two products need to be separated by HPLC.
  MitoPY1 Hydrogen peroxide Attaching a hydrophobic cation to a phenylboronate ester. It also reacts with peroxynitrite.
  MitoAR MitoHR Hydroxyl radical Reaction with hydroxyl radical cleaves off the quenching motif. They also react with peroxynitrite.
Mitochondrial inhibitors Rotenone Antimycin General ROS Rotenone is complex I inhibitor and antimycin is complex III inhibitor. Rotenone either decrease or increase ROS; Interruption of the ETC.
Mitochondrial antioxidant MitoQ MitoTEMPO MitoVitE General ROS Attaching a hydrophobic cation to antioxidants. -
Electron spin resonance MitoDEPMPO Free radicals Free radicals have unpaired electrons, and thus are paramagnetic and detectable by ESR. Lower sensitivity than fluorescent probes.