Figure 1

Antileukemic activity of silvestrol in vitro: (a) MV4-11 cells were incubated with 5 to 160 nM silvestrol. Cell viability was evaluated by a MTS assay at 24, 48 and 72 hours. (b) AML cell lines were incubated with 5 to 160 nM silvestrol, and viability was evaluated by a MTS assay 48 hours later. (c) AML primary cells unmutated FLT3 or with FLT3-ITD were incubated with 5 to 320 nM silvestrol. Cell viability was evaluated by MTS assay 48 hours following treatment. (d) Colony forming ability in primary blasts from FLT3-ITD positive and negative AML patients treated with 25 nM of silvestrol and scored 14 days later. (e) Primary blasts from FLT3-ITD positive and negative AML patients treated with 10, 30 and 50 nM of silvestrol for 48 hours and analyzed by annexin/PI flow cytometry. Values are presented as mean ± SEM.