Figure 3

Silvestrol down-regulates miR-155 and upregulates miR-155 target PU.1: (a) MV4-11 cells were treated with 50 nM of silvestrol and miR-155 expression was measured and normalized to U44 expression at 6, 12 and 24 hours following silvestrol treatment. (b) MV4-11 cells were treated with 50 nM of silvestrol for 24 hours, and miR-34a expression was measured thereafter. (c,d) MV4-11 cells were treated with 50 nM of silvestrol for 24 hours, and miR-155 (c) and PU.1 expression (d) levels were measured thereafter. (e,f) FLT3-ITD positive primary cells were treated with 50 nM of silvestrol for 24 hours, and miR-155 expression (e) and PU.1 expression (f) levels were measured thereafter. (g) MV4-11 and THP-1 cells were treated with 50 nM of silvestrol for 24 hours and assessed for P65 protein expression by immunoblotting. Values are presented as mean ± SEM.