Figure 4
In vivo antileukemic activity of silvestrol in FLT3 -ITD positive MV4-11 xenograft leukemia mouse model: (a) FLT3 expression by flow cytometry evaluated 48 hours after 1st and 2nd doses. (b) Spleen size after 3 silvestrol doses (day 6 of treatment) (c) Immunoblot of FLT3 protein expression in spleens from silvestrol-treated mice and vehicle-treated controls. (d) Cytospins of bone marrow cells and (e) histopathology of bone marrow (sternum), spleen, and liver from the silvestrol-treated and vehicle-treated leukemic mice and age-matched (non-leukemic) control mice showing the infiltration of leukemic blasts in the vehicle treated and signs of differentiation in the silvestrol treated mice (see red pointing arrows). Stain: H&E; magnifications, 400×. (f) Survival analysis of silvestrol-treated leukemic mice (N = 10) compared with the vehicle-treated controls (N = 6). Values are presented as mean ± SEM.