Figure 1

Identification and validation of PU.1 binding regions. (A) The visual representation of PU.1 targets on Chromosome 6 (chr6) partial regions identified through ChIP-seq analysis. The red and black tracks represent the ChIP-seq tag density of the sample (PU.1) and control (Input), respectively. (B) Validation of the PU.1 binding regions by ChIP-qPCR assays. Corresponding RefSeq genes for individual binding regions are marked underneath. Special codes “P” “E” “S” “L” inside the brackets, representing promoter, enhancer, short transcript and long transcript, were used to distinguish the regions corresponding to the same gene. (C) Luciferase reporter assays on representatives of PU.1 target genes (NCF4, NCF2, IL1B, BTK, PTPRC, NTS, RGS18, and CD1163) reporter plasmids and expression plasmids were co-transfected into 293T cells.