Figure 4

Hypoxia/HIF-1α may potentially promote proliferation of T-ALL cells via activation of the Notch1 signalling pathway. (A) Knockdown of HIF-1α or Notch1 expression significantly inhibited cell proliferation in hypoxic conditions. *, P < 0.01. Data plotted are mean ± SD of three separate experiments. (B) Similarly, cell cycle analysis showed a higher proportion of cells in the G0/G1 phase when HIF-1α or Notch1 is silenced compared with control. The percentage of cells in G1/G0, S and G2/M in each group is shown in (C). *, P < 0.01. Data plotted are mean ± SD of three separate experiments. (D) Representative Western blot analyses of G0/G1 phase cell cycle regulatory proteins in transfected cells cultured in hypoxia for 48 h.