Figure 5
Hypoxia promoted T-ALL invasion by transcriptionally up-regulating MMP2 and MMP9. (A) 104 cells were seeded onto matrigel-coated transwell inserts and allowed to migrate through the filter in normoxia or hypoxia for 48 h. Migrated cells were fixed, stained and captured at 100× and 400× magnification using the camera on an inverted microscope. (B) The total number of migrated cells was counted manually. Results shown are expressed as a percentage of control invasion. *, P < 0.01. Data plotted are mean ± SD of three separate experiments. (C) qRT-PCR showed that MMP2 and MMP9 were transcriptionally up-regulated in Jurkat and Sup-T1 cells kept in hypoxia for 48 h. The results normalized to β-Actin are presented as relative expression level. *, P < 0.01. Data plotted are mean ± SD of three separate experiments. (D) Western blot showing up-regulated protein levels of MMP2 and MMP9 in hypoxia. Results shown are representative of at least three independent experiments.