Figure 7
Hypoxia resulted in significant resistance to dexamethasone in T-ALL cells. (A) Jurkat and Sup-T1 cells were treated with 1 μM dexamethasone in normoxic or hypoxic conditions for 48 h and subjected to Annexin V/PI staining for flow cytometry. The percentage of cells is shown in each quadrant. (B) The hypoxia group had a lower percentage of apoptotic cells than the normoxia group by quantitative analysis. *, P < 0.01. (C) Antiapoptotic proteins Bcl-2, Bcl-xL and cleaved caspase 9 (Cas 9), caspase 3 (Cas 3) and PARP were estimated by Western blot. Results shown are representative of at least three independent experiments.