Functional analysis of EGFRvIII/CAR+T cells. (A) Cytotoxic activity of EGFRvIII/CAR+ T cells. (Left) Target cells were EGFRvIII-expressing U87 cells which can be lysed by EGFRvIII/CAR+ T cells, not by GFP+ and NT T cells. (Right) Target cells were EGFRvIII-negative U87 cells, which can not be lysed by either EGFRvIII/CAR+ or control T cells. (B) Cytokine release of EGFRvIII/CAR+ T cells. Only EGFRvIII/CAR+ T cells released significant amount of IFN-γ when co-cultured with EGFRvIII-expressing U87 cells. No increased IFN-γ expression was detected when co-cultured with EGFRvIII-negative U87 cells, nor from control GFP+ and NT T cells. Results are the mean and the SD from experiments in triplicate. * indicate P<0.05.