Vasculogenesis is disrupted in hNRASD12 -expressing transgenic embryos. (A-B) Confocal images of trunk vasculature of living control; flk1:GFP (A) and hNRASD12;flk1:GFP embryos (B) at 28 hpf. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessel. (C-D) Mid-trunk transverse sections of control;flk1:GFP (C) and hNRASD12;flk1:GFP embryos (D) at 28 hpf stained by GFP antibody (green) and Phalloidin-TRITC (red). NT, neural tube; NC, notochord. (E-J) Confocal images of head vasculature of living control;flk1:GFP (E, G, I) and hNRASD12;flk1:GFP embryos at 28 hpf (F, H, J). LDA, lateral dorsal aorta; PHBC, primordial hindbrain channel; CCV, common cardinal vein; BA, basilar artery. The red dotted lines denote the boundaries of the CCVs and PCVs respectively.