Induction of HOTAIR Expression by Col-1. A) Total cell RNA was extracted from rBM 3-D ± Col-1 cultures of A549 cells. The RNA levels of HOTAIR were measured using qRT-PCR and normalized to the house-keeping gene 36B4. A fold change was obtained setting the values from rBM 3-D culture to one. B) Similar to part A except that the RNA levels of mouse HOTAIR were compared between rBM ± Col-1 3-D cultures of mK-ras-LE cells. C) Similar to part A except that the RNA levels of H19, MALAT1, and XIST were compared between rBM ± Col-1 3-D cultures of A549 cells. D) The RNA levels of HOTAIR were measured using qRT-PCR in rBM ± Col-1 3-D culture of A549 cells with or without an integrin α2β1 neutralizing antibody (mAb-α2β1). A fold change in the HOTAIR transcript was obtained by setting the values from the control IgG group (CTL IgG) to one. E) Similar to part D except that the experiments were carried out in mK-ras-LE cells. Mean and standard deviations were obtained from three independent experiments. * indicated a P value < 0.05 as determined using unpaired two tail student T test.