Skip to main content
Figure 2 | Journal of Hematology & Oncology

Figure 2

From: TFPIα and TFPIβ are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activity

Figure 2

PI-PLC treatment of Sum102 and MDA-MB-231 cells. Serum-starved cells were treated with SFM (untreated) or PI-PLC (1 U/mL) for 2 hours before the supernatant was removed and cells lysed or collected for flow cytometry analysis. Total and free TFPI antigen measured in the supernatant (A and F) and lysate (B) of untreated and PI-PLC treated Sum102 (A and B) and MDA-MB-231 (F) cells. Mean values + SD (n ≥ 8) of three to six independent experiments are presented. All statistical comparisons were conducted between PI-PLC treated samples and untreated controls. Flow cytometry analysis of untreated (black, solid) and PI-PLC treated (black, broken) Sum102 (C) and MDA-MB-231 (G) cells. One representative experiment of three is shown. (D) Fluorescence images of SFM + glycerol (untreated, left panel) and PI-PLC (middle panel) treated Sum102 cells stained with antibody against both isoforms of TFPI. The right panel shows a secondary antibody background control staining. One representative experiment of three is shown. (E) Western blot of supernatants from SFM + glycerol (untreated) and PI-PLC treated Sum102 cells. Samples were deglycosylated and incubated with a polyclonal anti-human TFPI antibody.

Back to article page