Figure 3
From: TFPIα and TFPIβ are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activity
Heparin treatment of Sum102 and MDA-MB-231 cells. Serum-starved cells were treated with SFM (untreated) or heparin (5 U/mL) for 2 hours or with PI-PLC + heparin (2+2 hours) before the supernatant was removed and cells lysed or collected for flow cytometry analysis. The supernatant was discarded after PI-PLC pre-treatment. Free and total TFPI antigen measured in the supernatant (A and C) and lysate (B and D) of untreated, heparin or PI-PLC + heparin treated Sum102 (A and B) and MDA-MB-231 (C and D) cells. Mean values + SD (n ≥ 9) of three to six independent experiments are presented. Statistical comparisons were conducted between heparin/PI-PLC+heparin treated samples and untreated controls. (E) Flow cytometry analysis of untreated (black, solid) and heparin treated (black, dotted) Sum102 (left panel) and MDA-MB-231 (right panel) cells. One representative experiment of three is shown.