TFPIα and TFPIβ are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activity

Table 1 Characterization of TFPI and TF in a selection of tumor derived breast cancer cell lines and normal cells

Cell types	Description/Subtype	Origin	Invasiveness (*In vitro)	TFPIα mRNA (RQ)	TFPIβ mRNA (RQ)	Relative Free TFPIα (secreted)	TF mRNA (RQ)	Relative fl^†TF (cell-associated)
SUM102	Breast epithelial, basal-like	Tumor (primary)	Invasive	1.85	1.02	1.41	129	105
MDA-MB-231	Breast epithelial, basal-like	Tumor (metastatic)	Invasive	0.87	0.46	0.31	894	705
MCF-7	Breast epithelial, luminal-like	Tumor (metastatic)	Non-invasive	0.01	0.02	0.04	0.45	0.96
SK-BR-3	Breast epithelial, luminal-like	Tumor (metastatic)	Non-invasive	3E-03	0.02	0.05	1.58	1.64
BT-474	Breast epithelial, luminal-like	Tumor (primary)	Non-invasive	8E-04	6E-03	n.d	2.63	1.32
ZR-75-1	Breast epithelial, luminal-like	Tumor (metastatic)	Non-invasive	7E-04	5E-03	n.d	3.55	2.33
SUM149	Breast epithelial, basal-like	Tumor (primary)	Invasive	2E-04	4E-03	n.d	4373	948
HCAEC	Coronary artery endothelial	Primary (normal)	Non-invasive	1.00	1.00	1.00	1.00	1.00
EaHy926	Umbilical vein endothelial	Transformed	Non-invasive	1.11	0.83	0.38	0.62	0.61
ME16C2	Breast epithelial, basal-like	Primary (immortalized)	Invasive	0.11	0.25	0.28	336	170

* from references [38–42].
RQ - relative quantification calculated using 18sRNA as endogenous control. All values were calculated against HCAEC expression as a normal endothelial reference.
^† full length.
n.d. - not detected, below lowest standard.
TFPI and TF mRNA and protein levels were measured using qRT-PCR and ELISA in the breast cancer cells and in the non-cancerous breast epithelial cells ME16C2 and umbilical vein endothelial cell line EaHy926. All values were compared to the primary human coronary artery endothelial cells HCAEC and presented as relative values, mRNA results by the comparative Ct method and antigen levels by direct comparison.

ISSN: 1756-8722