Figure 7

Fyn kinase facilitates MC-mediated melanoma tumor growth. ( A and B ) Role of Fyn kinase in MC on IgE-induced melanoma tumor growth. Wsh Rec WT and Wsh Rec Fyn −/− mice were treated with a single i.v. administration of saline (−IgE) or monoclonal anti-DNP IgE (750 ng/mouse; +IgE). Twenty four hours later, mice were s.c. inoculated with B16 melanoma cells (+B16) in one ear pinna and tumor weight was determined after four weeks of inoculation. Representative pictures of tumors from Wsh Rec WT and Wsh Rec Fyn−/− mice after four weeks of inoculation are shown in A. Results are shown as the mean tumor weight from Wsh Rec WT and Wsh Rec Fyn−/− mice. Mean ± SEM (n = 3-11). *, P < 0.05 compared with IgE-treated mice. #, P < 0.05 compared with Wsh Rec WT IgE-treated mice. ( C ) Histological analysis of tissue biopsies. Ear pinna sections (2.5 μm) were stained with H&E and TB. Blood vessels are indicated by arrows and MC by arrowheads. Pictures are representative images (n = 3-4). Scale bar = 20 μm. ( D ) Quantification of blood vessels in ear pinna from treated mice. Mean ± SEM (n = 3). #, P < 0.05 versus Wsh Rec WT + IgE + B16; ø, P < 0.001 versus Wsh Rec WT-IgE + B16; Δ, P < 0.01 versus Wsh Rec WT + IgE + B16.