Figure 5

Biological consequences of PPP2R5C silencing. Absorbance of PPP2R5C- siRNA799- and PPP2R5C-siRNA991-treated and control cells (32D-bcr-abl-WT,32D-Bcr-Abl T315I) at different time points as measured by the CCK-8 method. The results represent mean values of three independent experiments. *, p < 0.001 compared with scrambled non-silencing control RNA-treated cells. Induction of apoptosis by PPP2R5C suppression in 32D-bcr-abl-WT and 32D-Bcr-Abl T315I cells 72 h after nucleofection with PPP2R5C siRNA (3 μg). *, p < 0.001,**, p = 0.005, ***, p = 0.001 compared with non-silencing control RNA-treated cells.