Skip to main content
Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Autoantibodies to Ezrin are an early sign of pancreatic cancer in humans and in genetically engineered mouse models

Figure 1

SERPA analysis of KC and KPC serum reactivity against K8484 cell line 2DE map. Total lysates from the K8484 cell line were separated by 2DE as described in the Methods section. Samples were focused in the first dimension using a gradient spanning the indicated pH range, separated in the second dimension in 4-12% acrylamide gels and subsequently Blue Coomassie stained (A) or transferred to a nitrocellulose membrane and probed with mouse sera. Three representative Western blot images show the immunoreactivity of control (B), KC (C) or KPC (D) serum. Immunoreactive protein spots were determined for each serum by superimposition of immunoblot signal pattern with the spot pattern of the corresponding Blue Coomassie stained gel using the “ProFinder 2D” software. Numbered circles indicate immunoreactive proteins specifically recognized by KC and KPC sera and identified by MALDI-TOF MS. Immunoreactive protein names are listed in Additional file 1: Table S1.

Back to article page