Figure 1

Sodium selenite-induced, cell-proliferation inhibition and apoptosis in cultured HL60 cells. (A) Sodium selenite inhibited proliferation of HL60 leukemia cells. Cells were treated with varying concentrations of selenite for 24 h, and cell viability was detected using CCK-8 kits. Mean values of three repetitions were displayed, and *P < 0.05, when compared with untreated cells. (B) Sodium selenite induced apoptosis in HL60 leukemia cells. Cells were collected after exposure to varying concentrations of selenite for 24 h and labeled with Annexin-V-FITC/PI, and the apoptotic ratio was detected using flow cytometry. The results were representative of three repetitions, and *P < 0.05, when compared with untreated cells. (C) Selenite induced the release of cytochrome C and AIF from the mitochondria to the cytoplasm. After cells were treated with 20 μM of selenite for the indicated times, fractions of mitochondria and cytoplasm were separated, and the cytochrome C and AIF contents of each fraction were detected by western blotting. This experiment was repeated at least 3 times. (D) Sodium selenite (20 μM) induced nucleus fragmentation of HL60 cells at 24 h, which was detected by immunofluorescence after nuclear staining with DAPI. Scale bar: 10 μm. This experiment was repeated at least 3 times.