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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo

Figure 2

Selenite altered microtubule assembly. (A) Sodium selenite (20 μM) destroyed microtubule integrity. After exposure to 20 μM of selenite, HL60 and Jurkat cells were collected at varying times, and the insoluble and soluble fractions of tubulin were harvested and detected by western blotting. Cells that were treated with Colchicine and Taxol were used as positive controls. This experiment was repeated at least 3 times. (B) Microtubule depolymerization was detected by indirect immunofluorescence. The cells were harvested after selenite exposure for the indicated times, and microtubules were indirectly labeled with a β-tubulin primary antibody and FITC-conjugated secondary antibody. Scale bar: 10 μm. This experiment was repeated at least 3 times.

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