Figure 5

Selenite had inhibitory effects on a HL60 xenograft tumor model. 4-week-old mice were divided into two groups randomly and each group was marked and put into its own box. The two groups lived in the same context and were fed with the same food and water. Then HL60 cells were given subcutaneously. After 1 week and tumors were detectable, PBS and selenite(1.5 mg/kg/day) were given to the control and selenite-treated group every two days respectively. After each group was injected for 3 weeks, nude mice were sacrificed for analysis. (A) Tumor weight decreased after selenite exposure. *P < 0.05. (B) Selenite caused nuclear pyknosis in tumor cells. This experiment was repeated at least 3 times. (C) Spleen weight decreased after selenite exposure. *P < 0.05. (D) Immunohistochemical staining indicated CD33 positive leukemia cells in spleen were decreased after selenite treated. Scale bar represented 100 μm. This experiment was repeated at least 3 times. (E) Selenite induced microtubule depolymerization in tumor cells. Soluble and insoluble-tubulin fractions were separated as indicated above and detected by western blotting. This experiment was repeated at least 3 times. (F) Selenite had similar effects on the regulation of Cyclin B1 and Mcl-1 as it did in vitro. This experiment was repeated at least 3 times. (G) In situ labeling of apoptotic tumor cells using the TUNEL assay indicated the appearance of apoptotic cells after selenite treatment. Scale bar represented 100 μm. This experiment was repeated at least 3 times. (H) Immunohistochemical staining indicated that alterations in Cyclin B1 and Mcl-1 levels were similar to those in vitro. Scale bar represented 25 μm. This experiment was repeated at least 3 times.