Figure 1

Characterization of iPS cells. (A) General morphology of iPS cells under study. (B) Human iPS cells were cultured on chamber slides, fixed and stained with either a control IgG or antibodies to SOX2 and OCT4. DNA was stained with Hoechst. Representative images are shown. (C) Human iPS cells were fixed and stained with either IgG or antibodies to NANOG and SOX2. Cells were then processed for analysis by flow cytometry. Experiments were repeated for at least three times. (D) Human iPS cells were cultured in a differentiation medium as described in Experimental procedures. Representative images of embryonic stem cell sacs (ES-sacs) and neural differentiation are shown.