Figure 4

Time-dependent responses of human iPS, Tera-1, and BEAS2B cells to H ₂ O ₂ . (A) Human iPS cells were cultured under the feeder-free condition as described in Experimental procedures and treated with 100 μM H₂O₂ for the indicated time periods (h). The whole cell lysates were collected and equal amounts of lysates were blotted with antibodies to various cellular components as indicated. (B) Tera-1 cells were treated and analyzed as described in A. (C) BEAS-2B cells were treated and analyzed as described in A.