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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Differential responses to genotoxic agents between induced pluripotent stem cells and tumor cell lines

Figure 6

Roles of p38 and CK2 on p53S392phosphorylation. (A) Human iPS, Tera-1, or BEAS-2B cells were treated with 10 μM Cr(VI), 100 μM H2O2, or 250 nM Dox for 4 h with or without pretreatment with 10 μM SB203580 or 10 μM LiCl for one h. HeLa cells treated with 500 mM NaCl was used as a positive control for p38 activation. Whole cell lysates were collected and equal amounts of cell lysates were blotted with antibodies to various molecular components as indicated. (B). Different amounts of human iPS cell lysates from experiments described in (A) were used for Western blot to match (or normalize) the total p53 level as indicated. The p-p53S392 level was detected using the antibody to p-p53S392. (C). Human iPS or BEAS-2B cells were treated with 10 μM Cr(VI), 100 μM H2O2, or 250 nM Dox for 4 h with or without pretreatment with 10 μM TBBz for one h. The whole cell lysates were collected and equal amounts of cell lysates were blotted with antibodies to various molecular components as indicated.

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