Figure 4

G-CSF/anti-G-CSF mAb complexes expand CD11b⁺Gr-1⁺myeloid cells after cytoreductive therapy. (A) B6 mice were sublethally irradiated (600 rad) on day 0 and treated i.p. on day 1, day 3, and day 5 with G-CSF/anti-G-CSF mAb complexes (1 μg G-CSF plus 5 μg anti-G-CSF mAb), G-CSF alone (1 μg G-CSF), vehicle alone, or IL-15/sIL-15Rα complexes (1.5 μg mIL-15 plus 7 μg sIL-15Rα-Fc) (n = 2/group). (B) Spleen cells were harvested on day 7 and analyzed by FACS analysis, and the absolute number of cells was determined. (C) Flow cytometric data from 'B’. CD11b and Gr-1 staining (top row) was gated with a large FSS/SSC gate, while CD8 and CD4 staining (bottom row) was gated with a tight lymphocyte gate. (D) B6 mice were treated with cyclophosphamide (4 mg) on day 0 and treated i.p. on day 1, day 3, and day 5 with G-CSF/anti-G-CSF mAb complexes (1 μg G-CSF plus 5 μg anti-G-CSF mAb), G-CSF alone (1 μg G-CSF), or vehicle alone (n = 4/group). (E) The percentage of CD11b⁺Gr-1⁺ within the peripheral blood. All data are representative of 2 independent experiments.