Figure 4

Modulation of CSC markers and miRNAs accompanies EMT of NSCLC cells. (A) A549M cells exhibit increased expression of CSC markers Sox2, Nanog and EpCAM and GDC-0449 inhibited such TGF-β-induced expression of CSC markers. TGF-β1-induced EMT also involved changes in the expression levels of (B) miR-200 family and (C) let-7 family of miRNAs. RNU6B and RNU48 were used as miRNA controls against which the data was normalized. *p<0.05 and **p<0.01.