Figure 5

Mechanistic role of miRNAs in TGF-β1 induced drug resistance. (A) Re-expression of miR-200s and let-7s sensitized A549M cells to erlotinib treatment. (B) Data from Figure 5A was used to calculate the extent of sensitization by re-expression of miRNAs upon erlotinib treatment, as measured by % inhibition of A549M resistance compared to parental A549 cells. (C) Re-expression of miR-200b+let-7c reversed EMT. E-cadherin and ZEB1 mRNA levels were determined by real time RT-PCR using GAPDH as the internal control. All the plotted values in Figure 5A are relative to vehicle-treated A549 cells. RNU6B and RNU48 were used as miRNA controls against which the data was normalized. *p<0.05.