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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Inhibition of Hedgehog signaling sensitizes NSCLC cells to standard therapies through modulation of EMT-regulating miRNAs

Figure 6

Mechanistic role of miRNAs in GDC-0449 effects on drug resistance. (A) GDC-0449 up-regulated the expression of miR-200b and let-7c, the two top regulated miRNAs in their respective families. (B) Down-regulation of miR-200 and let-7 family miRNAs abrogated the GDC-0449 mediated inhibition of erlotinib resistance in A549M cells. (C) Data from Figure 6B was used to calculate the abrogation of GDC-0449 effect through the use of anti-miRNAs, as measured by % inhibition of A549M sensitivity to GDC-0449, compared to control A549M cells. All the plotted values in Figure 6B are relative to vehicle-treated A549M cells and the first bar in the same figure represents A549M cells treated only with 10μM erlotinib. RNU6B and RNU48 were used as miRNA controls against which the data was normalized. *p<0.05.

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