Figure 1

Purification and in vitro activity of TAT-SALL4B protein. (a): Schematic diagram of SALL4B construct for TAT-SALL4B fusion protein expression in insect cells. (b): SDS-PAGE gel picture of Purified TAT-SALL4B, the size of TAT-SALL4B is ~95 KD. (c): Western blots showed TAT-SALL4B fusion protein could be recognized by SALL4 and or Histidine (His) antibodies. (d): TAT-SALL4B protein regulates Oct4 and SALL4 promoter activities as showed in a luciferase report assay. Protein concentrations were 5 nM (OCT4 promoter) and 10 nM (SALL4-promoter) separately. WT RLA was standardized to 1. (e): The inhibitory effect of TAT-SALL4B protein on SALL4 promoter activity is dose dependent. Note: Fold changes compared to WT were labeled on top of the diagrams. RLA: Relative Luciferase Activity; WT: Wide Type. (f): TAT-SALL4B protein promotes the proliferation of mouse LSK cells in culture.