Figure 6

Effect of miR-363-5p levels in the communication between endothelial cells:hematopoietic progenitor cells. (A) Adhesion of CD34+ cells to HUVEC 24 h post-transfection with scramble control, anti-miR-363-5p or pre-miR-363-5p with or without the addition of recombinant stem cell factor (SCF). Error bars represent s.e.m. of adherent cells average by field (20x magnification) counted by light microscopy in three replicates. ** P ≤ 0.01, ***P ≤ 0.001 by Student’s t test. (B) Number of colony-forming units on methylcellulose of CD34+ cells after a 24 h exposure to transfected HUVECs. Total colony number was counted after 7 days on methylcellulose. (C) Number of HUVEC, 48 h post-transfection with scramble control, anti-miR-363-5p or pre-miR-363-5p. Data represent the average of cell counting (20x magnification) by light microscopy ± s.e.m. in three replicates. *P ≤ 0.05 by Student’s t test. (D) Detection of SCF levels by ELISA in HUVEC conditioned media 48 h post-transfection with scramble control, anti-miR-363-5p or pre-miR-363-5p. Data represent the average of the SCF concentration from three transfection experiments ± s.e.m. *P ≤ 0.05 by Student’s t test.