Figure 3

Down-regulation of EGFR by EGFR siRNA in STS26T MPNST cells significantly decreased tumor cell proliferation, invasion, and migration by blocking the PI3K/AKT and MAPK pathways. (A) EGFR siRNA decreased EGFR expression and activation. Activated forms of PI3K/AKT and MAPK pathway factors decreased with EGFR inhibition. (B) EGFR siRNA significantly decreased tumor cell proliferation compared with the nonspecific control siRNA. (C, D) EGFR siRNA significantly decreased tumor cell invasion compared with the nonspecific control siRNA: cell invasion (C) and cell counts (D). (E, F) EGFR siRNA significantly decreased tumor cell migration compared with the nonspecific control siRNA: cell migration (E) and cell counts (F). * indicate P-values <0.05.