Figure 5
Stroma-promoted apoptosis protection of EL4, EL4-v10. (A-C) EL4/EL4-v10 (5×105) were seeded in triplicates on BSA or BM-Str or spleen-Str coated 24-well plates. Cell were cultured for 48 h in the presence of 20 μg/ml cisplatin; where indicated cultures contained 10 μg/ml IM7 or K926. EL4/EL4-v10 were thoroughly collected and apoptotic EL4 / EL4-v10 (AnnV+ or AnnV+/PI+) were determined by flow cytometry; (A,B) mean ± SD (triplicates) of % AnnV+ and AnnV+/PI+ cells and (C) representative examples; significant differences (p <0.05) in the presence of stroma: s, significant differences (p <0.05) in the presence of IM7 or K926: *. The experiment was repeated 3-times. BM- and spleen-Str exert a strong apoptosis protecting effect on EL4 and EL4-v10 that is neutralized by IM7, but not by K926.