Figure 8
Impact of EL4 and EL4-v10 on bone marrow stroma-promoted HSC apoptosis resistance. (A-D) HSC (1×106) were cocultured for 24 h-72 h with mitomycinC-treated EL4/EL4-v10 or EL4/EL4-v10 exosomes on BSA, HA or BM-Str-coated 24-well plates; where indicated, cultures contained 10 μg/ml cisplatin. (A-D) Apoptosis of HSC was evaluated by AnnV/PI staining and flow-cytometry (mean ± SD, triplicates and representative examples); (A) significant differences (p <0.05) by BM-Str: *; significant differences (p <0.05) in dependence on EL4 or EL4-v10: s; (C) significant differences (p <0.05) in the presence of antibody: *. Apoptosis-related protein expression was evaluated by flow-cytometry in HSC cultured overnight in the presence of 10 μg/ml cisplatin in 24-well plates (E-G) coated with BSA or BM-Str and (E,F) in the presence of EL4 or EL4-v10 exosomes (10 μg/ml) or (G) IM7 or K926 (10 μg/ml); (E-G) mean percent (triplicates) of stained HSC and representative examples; significant differences (p <0.05) by the presence of BM-Str: *, significant differences (p <0.05) by EL4/EL4-v10: s; significant differences (p <0.05) in the presence of antibody: *. Both EL4 and EL4-v10 interfere with the apoptosis-protective effect of BM-Str, which also is weakly affected by IM7.