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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Targeting xCT, a cystine-glutamate transporter induces apoptosis and tumor regression for KSHV/HIV-associated lymphoma

Figure 2

Targeting xCT induces apoptosis and death for KSHV-infected PEL cells. (A-B) KSHV-infected BC-1, BCP-1 and BCBL-1 cells were incubated with xCT inhibitor either Monosodium glutamate (MSG), or Sulfasalazine (SASP) at the indicated concentrations for 48 h, then cell viability was assessed by standard MTT assays. (C) BCP-1 or BCBL-1 were treated with MSG (20 mM), SASP (0.5 mM) or vehicle for 24 h, then proteins expression were measured by immuoblots. (D-E) BCP-1 or BCBL-1 were incubated with MSG or SASP at the indicated concentrations for 24 h (D), or transfected with negative control or xCT-siRNA for 48 h (E). Cell apoptosis was assessed using Annexin V-PI staining and flow cytometry analysis. Error bars represent the S.E.M. for 3 independent experiments. * = p < 0.05; ** = p < 0.01.

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