Figure 6

Inhibition of xCT induces viral lytic gene expression from KSHV-infected PEL cells. (A-B) BCP-1 (A) and BCBL-1 (B) cells were treated with either MSG (20 mM), SASP (0.5 mM) or vehicle for 24 h, then viral latent (Lana) and lytic gene (Rta, vGpcr, vIL-6, K8.1, Orf57) transcripts were quantified using qRT-PCR. Error bars represent the S.E.M for three independent experiments. (C-D) BCBL-1 were treated as above, then the expression of viral lytic protein K8.1 was detected by immunofluorescence and immunoblots. (E) BCBL-1 were treated by vehicle, MSG (20 mM), SASP (0.5 mM) and valproic acid (1.5 mM as a positive control), respectively, for 48 h, then the virion production were collected as described in Methods, followed by infection of HUVEC cells. Lana transcripts from each group were quantified by qRT-PCR. Error bars represent the S.E.M. for 3 independent experiments, ** = p < 0.01.