Figure 7
xCT inhibitor SASP suppresses PEL progression in vivo . (A-C) NOD/SCID mice were injected intraperitoneally (i.p.) with 107 BCBL-1 cells. Beginning 24 h later, 150 mg/kg SASP or vehicle (n = 8 per group) were administered intraperitoneally (i.p.) once daily, 5 days per week, for each of 2 independent experiments. Images of representative animals and their respective spleens, as well as ascites fluid volumes, were collected at the conclusion of experiments on day 28 (A/C). Weights were recorded weekly (B). Error bars represent the S.E.M. for 2 independent experiments, ** = p < 0.01. (D) Apoptosis was quantified by flow cytometry for live PEL cells purified from ascites fractions as described in Methods. (E) RNA was recovered from live PEL cells from ascites fractions from each of 4 mice representing vehicle- or drug-treated groups. qRT-PCR was used to quantify viral transcripts representing latent (Lana) or lytic genes (Rta, vGpcr, K8.1). Data were normalized to samples representing vehicle-treated mice.