Figure 4

GBR 401 induces homotypic adhesion and cell death in vitro. Raji, SU-DHL-6 and Mec-1 cell lines were incubated with mAbs (0.1 ng/mL – 5 μg/mL) for 2 hours. A/ Cell death was measured in flow cytometry by annexinV/7AAD. Flow cytometry panels are representative of at least three independent experiments (mAbs at a concentration of 1 μg/mL) and numbers indicate the percentage of dead cells (annexinV + 7AAD-, annexinV + 7AAD+, annexinV-7AAD+). The graphs show the percentage of dead cell +/- SD of three independent experiments. B/ Cell depletion after mAb incubation was assessed with CountBright™ Absolute Counting Beads. The graphs represent the absolute alive cell number +/- SD (annexinV-7AAD-) counted by flow cytometry experiment. C/ The clonogenic capacities of Raji and SU-DH-L6 cell lines were assessed with CFU assays. 500 cells were treated with the mAbs (1 μg/mL) for 2 hours and plated on methylcellulose medium containing proliferation factors. Fourteen days later, the colonies were counted under an inverted microscope. The graph shows the mean +/- SD of three independent experiments.