Figure 5

GBR401 does not trigger apoptosis and autophagy. Raji cells were pre-incubated with inhibitors of caspases (QVD, ZVAD), of lysosomal proteases (Chloroquine, Bafilomycin A), or of autophagosome formation (3MA) and were treated with mAbs (1 μg/mL) for 2 hours. Absolute alive cell numbers +/- SD and cell death +/- SD were then measured by CountBright™ Absolute Counting Beads and annexinV/7AAD flow cytometry staining.