Figure 2
Co-treatment with imatinib and TG101348 increased inhibition of cell growth and induced apoptosis of BCR-ABL-expressing cells in the presence of HS-5 cells. (A) K562 cells were cultured at a concentration of 8 × 104/mL in the presence or absence of HS-5 cells. Cells were then treated with varying concentrations of imatinib alone or in combination with TG101348 for 72 h. Viable cell numbers were calculated. Results are representative of three separate experiments. (B) K562 cells were treated with imatinib alone or in combination with TG101348 for 24 h, and total extracts were examined by immunoblotting using anti-phospho ABL, ABL, phospho-Crk-L, Crk-L, phospho-STAT5, STAT5, phospho-MAPK, Erk-1, cleaved PARP, and β-actin Abs. Blots were scanned and optical densities were determined using ImageJ software. (C) K562 cells were cultured with Z-VAD-fmk (20 μM) and/or TG101348 and imatinib for 24 h. Total extracts were analyzed by immunoblotting using anti-cleaved caspase-3 and cleaved PARP Abs. Actin was used as the loading control. Data are representative of two separate experiments. Blots were scanned and optical densities were determined using ImageJ software.