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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Synergistic effects of combined platelet-activating factor receptor and epidermal growth factor receptor targeting in ovarian cancer cells

Figure 1

WEB2086 and AG1478 exhibit synergistic cytotoxicity in CAOV-3 and SKOV-3 cells. (A) Under basal growth conditions, whole-cell extracts obtained from CAOV-3 and SKOV-3 cells were analyzed for EGFR and PAFR. (B) Detection of EGFR and PAFR expression by immunocytochemistry. 1, 2, 3 for CAOV-3 cells and 4, 5, 6 for SKOV-3 cells stained with anti-EGFR antibody, -PAFR antibody, and control IgG, respectively. (C) Cellular viability was measured by the CCK-8 assay. CAOV-3 and SKOV-3 cells were treated with the indicated concentrations of AG1478 and WEB2086 for 72 hours. (D) Isobologram analysis of combination WEB2086 with AG1478 used in equipotent concentrations in CAOV-3 and SKOV-3 cells. The line designates the CI where CI = 1 (additive effect). CI < 1 indicates synergism and CI > 1 represents antagonism. The combination data points (CI = 0.49 for CAOV-3 and CI = 0.58 for SKOV-3) calculated by CalcuSyn softwre indicate synergism at ED50. (E) The CI values of combination WEB2086 and AG1478 at a range of EDs. The CI at ED25, ED50, ED75 and ED90 indicate a synergistic interaction between WEB2086 and AG1478 in CAOV-3 and SKOV-3 cells.

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