Figure 3

Effects of WEB2086 and/or AG1478 on apoptosis in CAOV-3 and SKOV-3 cell lines. Apoptosis was evaluated as described in the Materials and Methods with Annexin V staining in CAOV-3 and SKOV-3 cells; the cells were treated with WEB2086 (0.1 mM), AG1478 (10 μM), and their combination. A and D: Representative dot plots illustrating the data near the mean of the groups in B and E. Columns, means of 3 identical wells of a single representative experiment; bars, upper 95% confidence interval; **, p < 0.005 for comparisons between cells treated with the combined treatment and cells treated with the single agent. C and F: Western blotting analysis of PARP and caspase-3 cleavage following treatment with WEB2086 alone or with AG1478. Western blotting of β-Actin is included as a loading control.