Expression profiling of the various WT1 transcripts in leukemia cell lines. (A) Map of the WT1 locus, showing the location of the various promoters, CpG islands and enhancer elements. Arrows represent direction of transcription. (B) Expression of WT1, AWT1 and WT1-AS in control bone marrow and peripheral blood leukocytes compared to hematological cancer cell lines. (C) The allelic expression of the various transcripts in expressing cell lines. (D) Western blot analysis of nuclear (N) and cytoplasmic (C) cellular fractionations reveal that the WT1 and AWT1 proteins are nuclear retained in leukemia cell lines. Purity of the cellular fractionations was confirmed using anti-sera against Nucleolin, a nuclear retained protein and cytoplasmic β-tubulin.