Figure 3

Histone ChIP in leukocytes and leukemia cell lines. The histone modification signature determined by ChIP-seq for (A) CD34+ and CD15+ cells. The levels of precipitation are comparable as the y-axis is limited to 50 reads for all tracks. The positions of the subsequent PCR amplicons used for ChIP are indicated. (B) ChIP specifically targeting the 5′ WT1 interval in normal leukocytes and myeloid derived cancer cell lines (C) K562 and (D) NB4. qPCR analysis with primers designed to the WT1 and AWT1 promoters performed on immunoprecipitated fractions and mock IPs. Graphs represent the percentage precipitation relative to input chromatin (mean values ± SEM).