Figure 5From: Argonaute 2 promotes myeloma angiogenesis via microRNA dysregulationmiR-92-1 directly targets ANGPTL1. The real-time PCR (A) and Western blot (B) detection of ANGPTL1 expression levels in LP-1-si-AGO2 cells after transfection with negative control and miR-92-1 mimics. (C) The effect of miR-92-1 on the luciferase activities of ANGPTL1 3′-UTR and ANGPTL1 3′-UTR with a mutated miR-92-1-binding site (mutant UTR, mUTR). miR-92-1 overexpression resulted in a significant decrease in the pmirGLO-reporter vector luciferase activity with ANGPTL1 3′-UTR but not with the vector containing the corresponding mutant.Back to article page