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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Argonaute 2 promotes myeloma angiogenesis via microRNA dysregulation

Figure 6

miR-145 directly targets VEGF. The real-time PCR (A) and Western blot (B) detection of VEGF expression levels in U266-pcDNA3-AGO2 cells after transfection with a negative control and miR-145 mimics. (C) The effect of miR-145 on the luciferase activities of VEGF 3′-UTR or VEGF 3′-UTR with a mutated miR-145-binding site (mUTR). miR-145 overexpression resulted in a significant decrease in the luciferase activity of the pmirGLO-reporter vector containing the VEGF 3′-UTR but not of the vector containing the corresponding mutant.

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